Title Eukaryotes from the hepatopancreas of lobster phyllosoma larvae
Authers Nobuaki SUZUKI, Keisuke MURAKAMI, Haruko TAKEYAMA and Seinen CHOW
Keywords prey organisms, phyllosoma larvae, Panulirus, scyllarid, 18SrDNA
Citation Bull. Fish. Res. Agen. No. 20, 1-7, 2007
Abstract
In order to investigate unknown prey organisms for lobster phyllosoma larvae, molecular approach was developed and adopted using the laboratory-reared and wild phyllosoma samples. The central domain of 18S rDNA was amplified via nested PCR using crude DNAs extracted from the hepatopancrea of the larvae and cloned into plasmid. Clones having 18S rDNA different from the hosts were screened by RFLP analysis, and the nucleotide sequences determined were subjected to FASTA homology search and phylogenetic analysis to identify the source of eukaryotic organisms. Feasibility of this method was confirmed in the laboratory-reared larvae of the Japanese spiny lobster (Panulirus japonicus) fed exclusively on either gonad of common mussel (Mytilus edulis) or Artemia nauplii. From the two wild-caught larvae (Scyllarus sp. and P. japonicus), 178 and 168 clones were isolated, of which 132 and 4 clones had different restriction profiles from the hosts, respectively. DNAs of Cnidaria and Urochordata were observed from the scyllarid larva, while those of fungi and host variant were observed in P. japonicus larva.
URI http://www.fra.affrc.go.jp/bulletin/bull/bull20/01.pdf