Title Novel molecular approach to study moulting in crustaceans
Authers Anna KUBALLA, Abigail ELIZUR
Keywords moulting, crustacean, microarray
Citation Bull. Fish. Res. Agen. No. 20, 53-57, 2007
Abstract
Moulting occurs in all arthropods, from insects to crustaceans, it is essential for growth, reproduction and metamorphosis. Moulting occurs in cycles and involves the shedding of the hard exoskeleton to expose a soft new shell, the uptake of water from the animals’ immediate surroundings causing the new exoskeleton to expand, and finally the hardening of the new exoskeleton. Moulting is a complex process that is affected by a range of external factors such as temperature, photoperiod, nutrition and eyestalk ablation. However despite extensive research the moulting process in crustaceans still remains poorly understood. Microarray technology provides a powerful, holistic approach to study gene expression in relation to changing physiological states. It enables not only the ability to profile the expression of genes already known to be involved in moulting, but also facilitates the discovery of new, as yet unknown, genes that may be important in the moulting process.
Understanding, and consequently controlling, the process of moulting, has significant potential for a range of commercial applications in crustaceans, such as the propagation of valuable seafood products. There are three areas within moulting control that have been identified as having potential commercial significance:
1. controlling the timing of the moult
2. manipulating the synchrony of moulting within a population (mass moulting)
3. controlling the process of shell hardening.
URI http://www.fra.affrc.go.jp/bulletin/bull/bull20/08.pdf