Title The Molecular Mechanism of Stress-Induced Apoptosis in Fish Cells and Embryos :
Activation of Neutral Sphingomyelinase-Ceramide Pathway
Authers Takeshi YABU and Michiaki YAMASHITA
Keywords apoptosis, heat shock, UV irradiation, gamma-ray irradiation, ceramide, neutral sphingomyelinase, caspase-3, zebrafish, embryo, Japanese flounder.
Citation Bull. Fish. Res. Agen. No.26, 15-21, 2008
Mechanism of stress-induced apoptosis in fish cultured cells and embryos has been characterized to be regulated by Mg2+-dependent neutral sphingomyelinase 1. The enzyme hydrolyzes sphingomyelin as a substrate and produces ceramide by its activation through various environmental stimuli, such as heat shock, UV irradiation, gamma-ray irradiation, and C2-ceramide treatment. Such ceramide signaling activates a pro-apoptotic caspase cascade and apoptosis characterized by DNA fragmentation, and resulted in abnormal morphogenesis in fish embryogenesis. Therefore, ceramide signaling functions as the important pro-apoptotic signal in fish development and apoptosis.
URI http://www.fra.affrc.go.jp/bulletin/bull/bull26/yabu1.pdf