Title Screening of superior trait-related genes of torafugu by using the genome database
Authers Shugo WATABE, Shigeharu KINOSHITA, Daisuke Ikeda, Satoshi Furukawa, Yuzuru SUZUKI, Kiyoshi KIKUCHI, and Wataru KAI
Keywords genome database, myosin heavy chain, QTL, sex determination, torafugu
Citation Bull. Fish. Res. Agen. No.26, 83-89, 2008
Torafugu Takifugu rubripes is an attractive vertebrate model organism for genomics by virtue of its smallest genome among vertebrates and available whole-genome database. Our group has constructed the torafugu linkage map with over 400 markers. In this study, we screened superior trait-related genes of torafugu by using the genome database and linkage map. Following two groups of genes were screened and analyzed, 1: Growth and muscle formation related genes, 2: Sex determining genes. (1) Quantitative trait loci (QTL) associated with high growth were screened by linkage analysis in crosses between a high-growth selected male and wild female. By using microsatellite markers, we identified several loci which may relate to the high-growth trait. We also analyzed genes (MYHs) encoding myosin heavy chain, the most important functional molecule among muscle proteins. A comprehensive analysis on the genome database revealed at least 28 MYHs in torafugu, almost twice as many as in humans. Expression analysis of each MYH showed their developmental stage-specific and tissue-specific expressions. (2) By performing linkage analyses, we showed that the sex of torafugu is determined by a single chromosomal region named SDY on linkage group 19 in an XX-XY system.
URI http://www.fra.affrc.go.jp/bulletin/bull/bull26/watanabe.pdf