Title Differentiation and maturation of sea urchin gonad
Authers Keisuke YAMANO, Takashi TODO, and Kazuhiro Ura
Keywords sea urchin, gonad, differentiation, maturation, vasa, nanos
Citation Bull. Fish. Res. Agen. No.26, 129-134, 2008
The process of differentiation and maturation of sea urchin gonads were studied at the molecular levels with the aim of controlling gonadal maturation. To find germ cell-specific makers, genes for vasa, PL10 and nanos were cloned. RT-PCR and in situ hybridization demonstrated that vasa and nanos genes were specifically expressed in germ cells in both sexes. Then, expression levels of those genes during embryonic development were measured by real-time PCR. Transcripts for vasa were accumulated in unfertilized eggs as a maternal factor whereas the expression of nanos gene enhanced during embryonic development. An ovary cDNA library was screened to find genes expressed with a stage-specific manner during ovarian maturation by the combination of EST and microarray analyses. Further analysis by real-time PCR on selected 18 genes demonstrated that 15 genes increased in their expression levels in the ovary during oogenesis. These genes encoded a component of cortical granules, cell division controlling factor, growth factor, enzyme, sperm-activation factor, unknown products, etc. We discuss about perspectives on the control of sea urchin maturation in relation to our study.
URI http://www.fra.affrc.go.jp/bulletin/bull/bull26/yamano.pdf