Title Availability of Genetically Modified Feed Ingredients for Rainbow Trout Oncorhnchus mykiss
Authers Shuichi SATOH and Pitchaya CHAINARK
Citation Bull. Fish. Res. Agen. No.31, 63-69, 2010
Abstract
The feeding studies were conducted to assemble the information on the availability of gene-modified (GM) feed ingredient for rainbow trout, using diet containing GM defatted soybean meal (SBM) as an alternative protein source to provide and ensure good protein accessibility and product safety. The utilization of genetically modified defatted soybean meal (GM SBM) as feed by rainbow trout was investigated, in comparison with non genetically modified defatted soybean meal (non-GM SBM). The nutrient utilization showed that there was no significant difference in growth and feed performance between GM and non-GM SBM groups in 12 week feeding experiment. However, the cauliflower mosaic virus 35S promoter fragment of the GM SBM was detected in the muscle of fish receiving GM SBM diet by Nested-PCR. Additionally, the promoter fragment vanished by the 5th day after changing the diet to non-GM diet. Subsequently, the study was carried out to examine the degradation and the possible carry over of foreign DNA fragment by means of measuring it from transgenic plant and host plant contained in GM or non-GM SBM and evaluate the safety for fish. These foreign DNA fragments were not completely degraded in stomach and intestine and might be taken up into organ via the garsrointestinal (GI) tract. However, foreign DNA was not detected after the withdrawal period. Judging from these findings, the novel feed ingredients derived from GM SBM could be considered as having equivalent nutritional quality and verifying the safety as feed ingredient.
URI http://www.fra.affrc.go.jp/bulletin/bull/bull31/31-3-2.pdf