Title Application of Physiological and Biochemical Methods to the Advanced Study of Reproductive Biology in Resource Fishes
Authers Akio SHIMIZU
Keywords reproductive physiology, endocrinology, ovary, post-ovulatory follicle,gonadotropin, vitellogenin, immunocytochemistry, reproductive parameter
Citation Bull. Fish. Res. Agen. supplement No. 4, 63-70, 2006
Abstract
Physiological and biochemical application to biology of resource fishes is an important matter for improving estimation and management of the resources. The female reproductive system is based on the integration of three systems; germ cell generating system (the ovary), the reproductive endocrine system, and the vitellogenic system. Specific chemical staining such as periodic acid-Schiff (PAS) reaction and immunochemical procedure such as immunostaining are convenient methods for detecting molecular markers of these systems. In the ayu, PAS staining evidently improved identification and detection of time-course changes of post-ovulatory follicles. In acanthopterygian fishes, universal antisera for immunocytochemical identification of FSH cells and LH cells have recently been obtained, and being successfully used for evaluating the activities of two different gonadotrophs in resource fishes such as mackerel, saury, and flounder. In the saury, antisera against vitellogenin can also be used for immunocytochemical evaluation of vitellogenenic activity. Further integrative studies on the three systems will be important for improving evaluation of biological properties of resource fishes.
URI http://www.fra.affrc.go.jp/bulletin/bull/bull-b4/10.pdf