Title Hormonal control of vitellogenin synthesis in the kuruma prawn Marsupenaeus japonicus
Authers Katsumi AIDA
Keywords kuruma prawn, vitellogenin, sinus gland, ovary, hepatopancreas
Citation Bull. Fish. Res. Agen. supplement No. 5, 21-24, 2006
Abstract
To develop new technologies for hormonal manipulation of shrimp reproduction, progress in the understanding of shrimp endocrinology is essential. In this study, complete cDNA sequence of vitellogenin(VTG, precursor of major yolk protein) of the kuruma prawn Marsupenaeus japonicus was determined, and quantitative reverse transcription-polymerase chain reaction(RT-PCR)method for VTG mRNA was developed. VTG mRNA levels in the ovaries and hepatopancreas were high during the ovarian development, indicating that both organs actively produce VTG. For screening hormonal activity, an in vitro assay method was developed by determining the effects of hormones on VTG mRNA in the incubated ovarian fragment and inhibitory effect of the sinus-gland peptide(SGP 3)was found. The developed in vitro hormone assay method with the RT-PCR are useful for screening shrimp hormones, and the inhibitory activity of SGP 3 can be utilized for hormonal manipulation of shrimp reproduction.
URI http://www.fra.affrc.go.jp/bulletin/bull/bull-b5/07.pdf