Title Genetic variation in artificial seed of Japanese abalone, Haliotis discus based on microsatellite DNA
Authers Motoyuki HARA and Masashi SEKINO
Keywords artificial seed, bottlenecked effect, genetic diversity, Haliotis discus, microsatellite DNA
Citation Bull. Fish. Res. Agen. supplement No. 5, 127-135, 2006
For evaluating the efficacy of the developed microsatellite DNA markers to detect changes of genetic diversity in artificial seed populations and to estimate the impact into natural populations due to stocking practice, we analyzed genetic variations within and between four artificial seed populations and four natural populations of Japanese abalone Haliotis discus containing Ezo-awabi and Kuro-awabi. All of 9 microsatellite loci screened in this study showed polymorphisms with highly variations. Marked reductions of genetic variability in artificial seed populations compared with the natural populations were observed in terms of number of microsatellite alleles. Significant differentiation was between the Ezo-awabi and Kuro-awabi, between the artificial seed populations and natural populations, and between the artificial seed populations. We conclude that, based on the decreased genetic variability observed in all the artificial seed populations examined, bottlenecked effects occurred when each artificial seed population was founded.
URI http://www.fra.affrc.go.jp/bulletin/bull/bull-b5/23.pdf