Title Genetic profiling of hatchery-raised Japanese flounder (Paralichthys olivaceus)based on DNA markers: an efficient means to track released flounder
Authers Masashi SEKINO
Keywords Japanese flounder (Paralichthys olivaceus), stock enhancement, microsatellites, mitochondrial DNA, parentage
Citation Bull. Fish. Res. Agen. supplement No. 5, 147-163, 2006
Abstract
This paper illustrates the efficiency of genetic profiling approach to determine a hatchery strain of Japanese flounder Paralichthys olivaceus, which was stocked into a natural sea area in a practical stock enhancement program. The hatchery strain tracked in this study was founded by using 18 wild captives in a hatchery station(JASFA hatchery), and the pedigrees of these offspring were identified by means of microsatellite DNA technique. In September 2001, the hatchery-raised fish(JASFA fish)were stocked into Miyako bay(Iwate Prefecture), and at around the same time and within the same area, a different hatchery strain was released by another hatchery station. In this study, microsatellites together with nucleotide sequences of the mitochondrial DNA control region were used as genetic tags to track the released JASFA fish. A total of 1,576 individuals including both wild and released fish were entrapped in a set net during a six month period one year after the release. Of these, 35 fish were retrieved as survivors of the released JASFA fish with the use of the two classes of molecular marker. The results presented here show that the genetic profiling would be of great use to further our understanding of stocking effects in Japanese flounder.
URI http://www.fra.affrc.go.jp/bulletin/bull/bull-b5/27.pdf